Schiff base method characterization of the newly synthesized Modified Levofloxacin Complex (MOLVX) and its activity against the HRT-18 cell line isolated from a male patient with colorectal adenocarcinoma

Abstract

Complexes have been synthesized using 10mL methanolic solution of raw levofloxacin (C18H20FN3O4) (0.0144g, 0.2mmol), 5mL methanolic solution of copper chloride dihydrate (II) (CuCl2_2H2O) (0.068g, 0.17mmol,), and 20mL methanolic solution of 1,10-phenanthroline(C12H8N2) (132mg, 0.2mmol). The complex was characterized by the Schiff base method for detection of the modification of the structure of levofloxacin using Hydrogen-Nuclear Magnetic Resonance H-NMR, Fourier Transform Infrared Spectroscopy (FT-IR), UV–visible (UV–vis) spectrophotometry, and elemental analysis of the complex using CHN analysis. The dried precipitate appears as light green crystals which are ground well by a clean spatula and weighed. Yield: 340 mg; 99%. This complex and levofloxacin have been subjected to melting point checking which was 268-270, and 221℃ respectively. Elemental analyses were achieved and were calculated for [Cu (Levo.) (Phen)Cl]. Cl, (C30H33Cl2CuFN5O4), molecular weight = 681.07 g/mol−1: C,53.29; H, 4.14; N, 10.36%.