SCE in human ‘metabolizing system’. The assessment of DNA damage caused by cytostatic therapy and its modification by a dihydroquinoline-type antioxidant

Abstract

The supposition that all eukaryotic cell types contain a plasma membrane (PM)-type Ca pump (i.e. a Ca pump which is directly-stimulated by calmodulin and located exclusively at the PM) has been questioned by recent data from higher plant cells. These studies suggest the presence of Ca pumps directly stimulated by calmodulin associated with an intracellular membrane (probably the endoplasmic reticulum, ER) in a variety of monocotelydonous and dicotelydonous species. Thus plants have a ‘PM-type’ Ca pump at an intracellular membrane. The evidence for this includes studies on isolated membranes, purification and functional reconstitution and phosphorylated intermediate formation. Plant cells also contain a homologue of the sarcoplasmic reticulum/endoplasmic reticulum (SRER) Ca pump, probably located at the ER. The implications of these new data for our appreciation of the structure, function and location of eukaryotic Ca pumps are discussed, together with recent data from the use of inhibitors specific to mammalian ERSR Ca pumps.