Abstract
Background: Staphylococcus aureus is a very important hospital and community-acquired pathogen that is prevalent in burn wounds, so finding the sources of contamination and infection with it are important for its control. The aim of this study was to do SCCmec typing and determine the prevalence of pvl, ACME, sea and seb genes among S. aureus isolates from burn patients. Methods: Fifty S. aureus isolates were sampled from burn patients and were identified. These isolates were typed based on SCCmec typing and detection of mecA and pvl genes using multiplex polymerase chain reaction (PCR) method and also were screened for the presence of ACME, sea and seb genes by PCR. Results: Three isolates were identified as methicillin-resistant strains. The sea gene was identified in 22% of isolates while pvl, ACME and seb genes were not found in the isolates. Multiplex PCR SCCmec typing of 3 methicillin resistant S. aureus (MRSA) isolates showed that 2 isolates belong to SCCmec type II and 1 isolate to type I. Conclusions: The results suggest that methicillin resistant S. aureus isolates are prevalent in burn patients and mecA gene is involved in resistance to methicillin. These strains belonged to SCCmec type II and I which can affect horizontal gene transfer among S. aureus isolates and hence distribution of virulence genes. Therefore, it is necessary to frequently monitor S. aureus isolates through typing and screening virulence genes to control this pathogen.
Highlights
Staphylococcus aureus is a very important hospital and community-acquired pathogen that is prevalent in burn wounds, so finding the sources of contamination and infection with it are important for its control
The aim of the study was to do staphylococcal cassette chromosome mec (SCCmec) typing and detect pvl, arginine catabolic mobile element (ACME), sea and seb genes in S. aureus isolates from patients admitted to a burn hospital in Ahvaz
Materials and Methods Bacterial Strains Fifty S. aureus isolates previously collected from burn patients in Taleghani hospital, Ahvaz, southwest of Iran were included in this study
Summary
Staphylococcus aureus is a very important hospital and community-acquired pathogen that is prevalent in burn wounds, so finding the sources of contamination and infection with it are important for its control. Methods: Fifty S. aureus isolates were sampled from burn patients and were identified These isolates were typed based on SCCmec typing and detection of mecA and pvl genes using multiplex polymerase chain reaction (PCR) method and were screened for the presence of ACME, sea and seb genes by PCR. Conclusions: The results suggest that methicillin resistant S. aureus isolates are prevalent in burn patients and mecA gene is involved in resistance to methicillin. These strains belonged to SCCmec type II and I which can affect horizontal gene transfer among S. aureus isolates and distribution of virulence genes. Detection of the SCCmec type of an isolate is necessary for control of hospital infections and prevention of the transmission of infections [6]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
