Scatter Factor/Hepatocyte Growth Factor Expression Enhances Human Glioblastoma Tumorigenicity and Growth

Abstract

We have shown previously that the multifunctional cytokine scatter factor/hepatocyte growth factor (SF/HGF) is elevated in human malignant gliomas. In this study we investigated how human SF/HGF expression affects the malignancy of the U373 human glioblastoma cell linein vivoandin vitro.Human SF/HGF gene transfer increased U373 glioblastoma tumorigenicity by ≥20-fold and enhanced the growth rate of intracerebral U373 xenografts by 3- to 8-fold. SF/HGF expression had no effect on the proliferation of glioblastoma cell monolayers but increased their anchorage-independent colony formation in soft agar by 5- to 8-fold. These results are the first to show that SF/HGF expression by human glioblastoma cells enhances their growth dysregulationin vitroand malignancyin vivo.