Abstract
AbstractSDS is widely used in sample preparation for proteomic research. However, SDS is incompatible with LC and electrospray ionization. SDS depletion is therefore required ahead of LC–MS analysis. Most of current SDS removal strategies are time consuming, laborious, and have low reproducibility. Here, we describe a method, SDS–cyclodextrin (CD)-assisted sample preparation, by which CD can bind to SDS and form CD–SDS complexes in solutions, allowing for direct tryptic digestion. We demonstrate that SDS–CD-assisted sample preparation is a simple, fast, and robust SDS-based sample preparation method for proteomics application.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
