Abstract
A scanning near-field optical microscope (SNOM) is applied to fluorescence imaging of biological samples in liquid, including live cells. The SNOM is mounted on a Zeiss Axiovert 135 TV fluorescence microscope. For feedback we use tuning fork shear force method. The scanning tip is produced from a 125 μm optical fibre (8.3 μm core diameter) in a commercial Sutter P-2000 pipette puller and is coated with aluminium. Other commercial tips have also been used. Coarse z-axis adjustment is hydraulic, and fine positioning is accomplished with piezoelectric tube units. We have constructed the original liquid chamber, which allows long term stability of scanning and highest values of the Q factor (300 or more). The depth of liquid layer was less than 40 μm. Near-field images – the topography and distribution of membrane fluorescence of live human epithelial A431 cells, stably transfected with an EGFP fusion protein of the epidermal growth factor transmembrane receptor protein (EGFR, erbB1), were obtained in liquid.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
