Abstract
The structure and microbial flora of the digestive tract of larval Simulium ornatum were investigated using scanning electron microscopy. Direct observations of the microbial communities associated with the endoperitrophic surface of the peritrophic membrane and the endocuticular surface of the hindgut are presented. The endoperitrophic surface was frequently devoid of bacteria, although the fungus Harpella melusinae (Harpellales, Trichomycetes) was commonly attached. Rarely, spirochaetes and coccoid bacteria were attached to the endoperitrophic surface. In contrast, the endocuticular surface was regularly colonized by a diverse microflora composed of rod-shaped, coccoid, spiral, and filamentous bacteria and two species of Harpellales (Trichomycetes). A freeze-fracture technique is compared with a dissection technique for exposing the endoperitrophic and endocuticular surfaces of the digestive tract.
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