Abstract
A technique for fracturing plant tissues to permit the examination of internal features of cells by scanning electron microscopy is described. Specimens were fractured fresh, without the use of cryoprotectants or chemical fixatives, macerated with a dilute solution of osmium tetroxide, and then critical-point-dried. Cytoskeletal components were observed in mesophyll cells of Japan Laurel, Aucuba japonica (Cornaceae), although they were absent in preparations using fixatives or cryoprotectants. They consisted of radiating arrays of fibrils associated with chloroplasts, plasma membranes, or aggregations of spherical bodies. In places the fibrils were seen to be bundles made up of finer strands of dimensions similar to microtubules.
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