Abstract
In addressing the challenge of inflorescence emergence in cytoplasmic male sterile line (CMS) rice, specifically the CMS A-line, GA3 has conventionally been utilized. However, the high cost of GA3 poses financial constraints for middle-class and marginal farmers. This study aims to identify cost-effective alternatives to GA3 for CMS breeding. Utilizing IR58025A as the A-line and KMR3 as the R-line, obtained from Rice Research Station, Chinsurah, Hooghly, the experiment was conducted following CMS breeding layout design at the Crop Research Farm under the Department of Botany, the University of Burdwan. Various chemicals, including GA3 , penicillin, sulfonamide, and gentamicin, were applied as foliar spray treatments, alongside a control group, during flowering to produce F1 seeds from the A-line in the boro season. The primary objectives include exploring microfeatures of different plant organs such as leaves, stems, androecium, and gynoecium through SEM analysis to evaluate the effectiveness of the diverse treatments. This research contributes valuable insights for rice breeders and the scientific community, offering potential alternatives to GA3 in CMS breeding technology.
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