Abstract
To obtain specimens suitable for viewing the early stages of development in Agaricus bisporus, we modified the Halbschalentest method. A plastic foam, impregnated with peat moss, enabled examination ofthe mycelium throughout its entire depth by scanning electron microscopy. Early in the developmental sequence, single hyphae with infrequent branches appeared. As development progressed, the hyphae were consolidated into strands by adhesive, mucilaginous substances. These substances formed electronlucent sheets which were especially obvious after the mycelium was watered. The initial stage of primordium formation was marked by appearance of hyphae which were turgid and tended to curve sharply and branch frequently. These hyphae were found in both initial and later stages of primordium development. Primordia that continued to increase in size appeared to recruit hyphae and strands from surrounding mycelium.
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