Abstract
Clubroot galls taken from 40-day-old Brassica rapa L. var. glabra Kitamura, grown in soil infested with Plasmodiophora brassicae Wor. were examined by scanning (SEM) and transmission (TEM) microscopy. SEM materials were prepared by freeze fracturing followed by ion etching. A thin layer enveloped the mass of sporangia which in etched specimens appeared to be embedded in a fibrillar net deposited among the sporangia. Both SEM and TEM micrographs revealed a circular germ pore (ca. 1 μm in diameter) with a thickened plug-like region in the pore orifice. The wall of the mature resting sporangium consisted of three layers W1, W2, and W3.
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