Scanning and transmission electron microscopy of the ependyma of the fourth ventricle in the monkey brain.

Abstract

The ependymal structure of the fourth ventricle floor of adult rhesus monkeys was investigated using both scanning and transmission electron microscopy. Each half of the floor exhibited dense ciliation which decreased towards the median sulcus. The area postrema and the facial colliculus lacked cilia and were covered with non-ciliated ependymal cells. Along the entire length of the median sulcus supraependymal globular protrusions were seen. Over the rostral and middle thirds of the sulcus these protrusions exhibited interwoven ridges. At the former situation they were closely approximated but at the latter they were on the surface of non-ciliated nodules which were lying amongst sparsely ciliated cells. In the caudal third these protrusions presented smoother surfaces without any structural details over them. Transmission electron microscopy through the upper two thirds of the median sulcus revealed these surface protrusions to be non-ciliated cells having long and profuse network of interwoven microvilli over their luminal surface. These cell bodies exhibited well-defined supranuclear Golgi complexes, vesiculated rough and smooth surfaced endoplasmic reticulum, free ribosomes and polyribosomes, few electron dense granules, network of microtubules and light and dense multivesiculated bodies. These features suggested a secretory and/or absorptive role, rather that autophagy to these surface specialization.