Abstract
Recent advances in optical coherence tomography (OCT)-based angiography have demonstrated a variety of biomedical applications in the diagnosis and therapeutic monitoring of diseases with vascular involvement. While promising, its imaging field of view (FOV) is however still limited (typically less than 9 mm2), which somehow slows down its clinical acceptance. In this paper, we report a high-speed spectral-domain OCT operating at 1310 nm to enable wide FOV up to 750 mm2. Using optical microangiography (OMAG) algorithm, we are able to map vascular networks within living biological tissues. Thanks to 2,048 pixel-array line scan InGaAs camera operating at 147 kHz scan rate, the system delivers a ranging depth of ~7.5 mm and provides wide-field OCT-based angiography at a single data acquisition. We implement two imaging modes (i.e., wide-field mode and high-resolution mode) in the OCT system, which gives highly scalable FOV with flexible lateral resolution. We demonstrate scalable wide-field vascular imaging for multiple finger nail beds in human and whole brain in mice with skull left intact at a single 3D scan, promising new opportunities for wide-field OCT-based angiography for many clinical applications.
Highlights
The visualization of functional vascular networks and architecture of blood vessels within living biological tissue plays a critical role in the diagnosis and therapeutic monitoring of diseases, as well as in the study of tissue pathology and morphology [1]
The smallest pattern that can be resolved by the wide field of view (FOV) mode is the element 3 in the group 4, as pointed by the red arrow, upon which the full width at half maximum (FWHM) contrast of the line width is measued at 24.8 μm
We demonstrated an unprecedented field of view up to 750 mm2 for vascular imaging at one single 3D acquisition
Summary
The visualization of functional vascular networks and architecture of blood vessels within living biological tissue plays a critical role in the diagnosis and therapeutic monitoring of diseases, as well as in the study of tissue pathology and morphology [1]. The imaging modalities, e.g., confocal, multiphoton and fluorescence microscopy and scanning electron microscopic imaging, allow for cellular structural and functional visualization to elucidate abnormalities of vessel angiogenesis and remodeling [2,3,4] These microscopy-based imaging methods are capable of achieving superior spatial resolution, ranging from a few nanometers to submicrons, the shallow imaging depth and small field of view (FOV) often limit their applications to small specimens or preserved samples that require laborious tissue biopsy and preparation. Due to the performance of light source (mainly the wavelength and spectral bandwidth), OCT can achieve micron-scale axial resolution (
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