Scalable, Lightweight, Integrated and Quick-to-Assemble (SLIQ) Hyperdrives for Functional Circuit Dissection.

Li Liang,Stefan N Oline,Justin C Kirk,Robert W Komorowski,Lukas Ian Schmitt,Miguel Remondes,Michael M Halassa

doi:10.3389/fncir.2017.00008

Abstract

Independently adjustable multielectrode arrays are routinely used to interrogate neuronal circuit function, enabling chronic in vivo monitoring of neuronal ensembles in freely behaving animals at a single-cell, single spike resolution. Despite the importance of this approach, its widespread use is limited by highly specialized design and fabrication methods. To address this, we have developed a Scalable, Lightweight, Integrated and Quick-to-assemble multielectrode array platform. This platform additionally integrates optical fibers with independently adjustable electrodes to allow simultaneous single unit recordings and circuit-specific optogenetic targeting and/or manipulation. In current designs, the fully assembled platforms are scalable from 2 to 32 microdrives, and yet range 1–3 g, light enough for small animals. Here, we describe the design process starting from intent in computer-aided design, parameter testing through finite element analysis and experimental means, and implementation of various applications across mice and rats. Combined, our methods may expand the utility of multielectrode recordings and their continued integration with other tools enabling functional dissection of intact neural circuits.

Highlights

Experimental access to neural circuits has progressed rapidly with recent developments in optical and molecular tools
For a multi-electrode array to be used with freely moving small rodents such as the mouse, it must be designed within the boundaries of certain constraints (Figure 1)
A key feature of the SLIQ hyperdrive is the ability to scale the number of microdrives as needed from 2 to 32, and here we provide suggested parameter values for several versions (Figure 1D)

Summary

Introduction

Experimental access to neural circuits has progressed rapidly with recent developments in optical and molecular tools. SLIQ Hyperdrives for Circuit Dissection rodents, require expertise to make design alterations, or trade weight for mechanical stability To overcome these challenges, we developed a Scalable, Lightweight, Integrated and Quick-toassemble (SLIQ) multielectrode array platform that minimizes weight but maximizes structural stability. We developed a Scalable, Lightweight, Integrated and Quick-toassemble (SLIQ) multielectrode array platform that minimizes weight but maximizes structural stability This platform employs a novel ‘microdrive’ design that enables adjustment of electrode position based on a spring and screw mechanism. Both the design of individual microdrives as well as that of its parent platform can be adjusted based on experimental needs, enabling functional circuit dissection across different models and species

Methods

Results

Conclusion