SBSE(EG-Silicone)-UFLC-MS/MS and HPLC-Flu Analysis of Norharman in Passiflora edulis Rinds

Vítor Freire,Janete Yariwake,Renata Colombo

doi:10.21577/0103-5053.20190136

Vítor Freire, Janete Yariwake + Show 1 more

Open Access

https://doi.org/10.21577/0103-5053.20190136

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Abstract

Previous studies on Passiflora edulis (sour passion fruit) rinds reported some biological activities and there is a growing interest on rinds flour as a possible functional food, but its alkaloid composition was not detailed investigated. This work reports on for the first time in the literature the identification of norharman in P. edulis rinds, by using stir bar sorptive extraction combined with ultra-fast liquid chromatography coupled to tandem mass spectrometry [SBSE(EG-Silicone)-UFLC-MS/MS)]. This β-carboline alkaloid is suspected to be neurotoxic. Therefore, another purpose of this study was to develop methods of targeted quantification of norharman in P. edulis rinds extracts using polydimethylsiloxane as stationary phase (SBSE(PDMS)) and a copolymer of polydimethylsiloxane and polyethylene glycol as stationary phase (SBSE(EG-Silicone)) combined with high performance liquid chromatography with fluorescence detection (HPLC-Flu) and ultra-fast liquid chromatography coupled with tandem mass spectrometry (UFLC-MS/MS). Norharman extraction by SBSE(PDMS) and SBSE(EG-Silicone) was optimized and compared, and the analytical performance of SBSE(EG-Silicone) method was superior to that of SBSE(PDMS). The analysis of a sample of dried P. edulis rind indicated (332.16 ± 8.43) pg g-1 of norharman.

Highlights

Passion fruit (Passiflora edulis) is a tropical fruit, widely consumed and distributed around the world
Considering the potentially toxic effects of β-carboline alkaloids, this study focuses on the targeted analysis of norharman in Passiflora edulis rinds, using stir bar sorptive extraction (SBSE), HPLC-Flu and UFLC‐mass spectrometry (MS)/MS, in the SRM mode
Lamounier et al.[39] report the quantification of norharmane into infusions prepared with commercial samples of “passion fruit tea”, containing P. alata Dryander, by micelar liquid chromatography-fluorimetric detection (218 ng of norharmane + 144 ng of harmine in tea infusion prepared with two tea packages). Their results suggest that phytotherapic preparations containing Passiflora should be deeper investigated. This is the first report of norharman in P. edulis rinds using a SBSE(EG-Silicone)-UFLC-MS/MS method

Summary

Introduction

Passion fruit (Passiflora edulis) is a tropical fruit, widely consumed and distributed around the world. Considering the potentially toxic effects of β-carboline alkaloids, this study focuses on the targeted analysis of norharman (identification and quantification) in Passiflora edulis rinds, using SBSE, HPLC-Flu and UFLC‐MS/MS, in the SRM mode.

Results

Conclusion