SBA-15 Mesoporous Silica as Delivery Vehicle for rhBMP-2 Bone Morphogenic Protein for Dental Applications.

Dimitrios Gkiliopoulos,Konstantinos Xanthopoulos,Evangelia Stalika,Georgia K Pouroutzidou,Konstantinos Triantafyllidis,Eleana Kontonasaki,Evi Christodoulou,Ioannis Tsamesidis,Anna Theocharidou,Dimitrios Bikiaris

doi:10.3390/nano12050822

Dimitrios Gkiliopoulos, Konstantinos Xanthopoulos + Show 8 more

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https://doi.org/10.3390/nano12050822

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Journal: Nanomaterials	Publication Date: Feb 28, 2022
Citations: 12	License type: CC BY 4.0

Affiliation: Aristotle University of Thessaloniki

Abstract

(1) Background: A proposed approach to promote periodontal tissue regeneration in cases of peri-implantitis is the local administration of growth factors at the implant site. Recombinant human bone morphogenetic protein-2 (rh-BMP-2) can effectively promote bone regeneration and osseointegration and the development of appropriate carriers for its delivery is of paramount importance. The aim of the present study was to develop SBA-15 mesoporous nanoparticles (MSNs) with varying porosity, evaluate their biocompatibility with human Periodontal Ligament Cells (hPDLCs) and to investigate their effectiveness as carriers of rh-BMP-2. (2) Methods: SBA-15 type mesoporous silicas were synthesized via sol–gel reaction. The calcined SBA-15 samples were characterized by N2 porosimetry, Fourier transform–infrared spectrometry (FTIR), Scanning (SEM) and Transmission Electron Microscopy (TEM). Rh-BMP-2 loading and release kinetics were evaluated by UV spectroscopy. (3) Results: MSNs presented hexagonally arranged, tubular pores of varying length and diameter. Slightly higher loading capacity was achieved for SBA-15 with large pores that presented good hemocompatibility. MTT assay revealed no cytotoxic effects for all the tested materials, while SBA-15 with large pores induced a significant upregulation of cell viability at day 5. (4) Conclusions: SBA-15 MSNs may prove a valuable delivery platform towards the effective release of bone-inducing proteins.

Highlights

IntroductionMesoporous silica is a class of materials containing periodic arrays of pores, that have tunable size over the range of 2–50 nm [1]
Considering that drug delivery systems are critical for the function of recombinant human BMP-2 (rhBMP-2), the aim of the present study was to develop SBA-15 mesoporous nanoparticles with varying porosity, evaluate their hemocompatibility and viability with human Periodontal Ligament Cells and to investigate their effectiveness as carriers of rhBMP-2
Once we demonstrated the hemocompatibility of both SBA‐15 Mesoporous silica nanomaterials (MSNs), we elected the contact with human Periodontal Ligament Cells (hPDLCs)

Summary

Introduction

Mesoporous silica is a class of materials containing periodic arrays of pores, that have tunable size over the range of 2–50 nm [1]. Mesoporous silica nanomaterials (MSNs) gained a raised interest in various applications, such as catalysis [2], water treatment [3], drug delivery [4–7], etc., due to their production via varying and relatively simple methodologies [8], special structural features, extensive multifunctionality and facile functionalization [9]. SBA-15 (Santa Barbara Amorphous) is a well-ordered MSN with hexagonal pore arrangement and uniform pore sizes up to 30 nm [10,11]. As a large pore MSN, SBA-15 is an ideal carrier of large biomolecules (e.g., proteins), that combines high biomolecule loadings, biocompatibility, capacity for both hydrophobic and hydrophilic proteins with various

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