介孔分子筛SBA-15固定化脂肪酶性质的研究<br>Immobilization of Lipase on Mesoporous SBA-15 and Its Characterization

Abstract

本文以介孔分子筛SBA-15为载体采用吸附法对假单胞菌脂肪酶进行了固定化，研究了固定化条件对酶的活性及酶活回收率的影响，得到了最佳固定化条件。并对固定化酶的稳定性(pH、操作稳定性以及储藏稳定性)做了初步研究。结果表明：当载体为1 g，给酶量为50 mg，固定化时间为3 h，溶液的pH值为8.0时，假单胞菌脂肪酶的固定化效果最好，此时酶活力回收率达到60%左右。固定化酶的稳定性较游离酶有很大提高。研究结果表明介孔材料SBA-15是脂肪酶进行固定化的一个优良载体。 Lipase was immobilized on the mesoporous molecular sieve SBA-15 by physical adsorption method. The effects of immobilization conditions on the relative activity and the activity yield of the immobilized lipase were studied. At the same time the pH, operational and storage stability of the immobilized lipase was discussed. The results showed that the optimum conditions for immobilization were as follows: the mass of the carrier, the mass of the free enzyme, time and pH were 1 g. 50 mg, 3.0 h and 8.0, respectively. Under the optimum condition, the activity yield of the immobilized lipase was around 60%. The immobilized lipase showed a good stability compared with the free enzyme. The results showed that the mesoporous molecular sieve SBA-15 is an excellent carrier for the immobilization of lipase.