Sazlıdere Baraj Gölünde Yaşayan Balık Türlerinde Aeromonas salmonicida Varlığının Araştırılması

Abstract

Objective: The aim of this study was, to examine the presence of Aeromonas salmonicida in lake water and fish species living in Lake Sazlıdere, a region where fish hunting was also conducted, in Istanbul. Material and Methods: In this study, between April 2014 and March 2015, samples from Lake Sazlıdere were collected for once a month. Samples were divided into three groups including, swabs materials from fish surface (n=48), viscera (n=46) and lake water (n=60). The samples inoculated on to the Furunculosis Agar and Tryptic Soy Agar including Coomassie Brilliant Blue (C-TSA) and incubated at 25°C for 7 days. Presumptive colonies were examined with biochemical tests for the identification of A. salmonicida. Concurrently with bacteriological examination, all of the samples were examined for the presence of vapA gene with Polymerase Chain Reaction (PCR). Results: According to bacteriological examination results, A. salmonicida were not isolated from any of the samples. As a result of PCR, of 60 water samples 3 (5.0%) were positive for vapA gene, while A. salmonicida specific DNA was not detected in the viscere and swab samples. Conclusion: It has been determined that infections caused by A. salmonicida, especially furunculosis disease, under this light do not constitute a risk in Lake Sazlıdere at the time of the study. However, the information obtained from fishermen and the results of PCR, when evaluated together with chemical contamination in general, indicate that continual investigations should be continued in this area, not within a certain period of time.