Abstract

Superresolution microscopy based on stimulated Raman scattering (SRS) is appealing for label-free quantitative imaging of biological and biomedical systems with higher resolution than conventional SRS microscopy, which is diffraction-limited. This study experimentally demonstrates a saturated SRS (SSRS) technique to break the diffraction limit for vibrational imaging of biological samples, such as chloroplasts and Hela cells. The technique holds great promise for studying living cells.

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