Saturated fatty acid attenuates anti-obesity effect of green tea

Shuya Yamashita,Mai Kadomatsu,Kanami Nakahara,Hirofumi Tachibana,Ai Hikida,Shuhei Yamada,Yuka Sato,Asami Hirashima,Konatsu Fujino,Kyosuke Murata,Mari Maeda-Yamamoto,Ayaka Nezu,Ren Yoshitomi,Motofumi Kumazoe,Jaehoon Bae,Motoki Murata,I-Chian Lin

doi:10.1038/s41598-018-28338-5

Abstract

Green tea and its major polyphenol epigallocatechin-3-O-gallate (EGCG) have suppressive effect on dietary obesity. However, it remains unsolved what type of diet on which they exhibit high or low anti-obesity effect. In the present study, we investigated whether anti-obesity effect of green tea differs depending on composition of fats or fatty acids that consist high-fat (HF) diet in mouse model. Green tea extract (GTE) intake dramatically suppressed weight gain and fat accumulation induced by olive oil-based HF diet, whereas the effects on those induced by beef tallow-based HF diet were weak. GTE also effectively suppressed obesity induced by unsaturated fatty acid-enriched HF diet with the stronger effect compared with that induced by saturated fatty acid-enriched HF diet. These differences would be associated with the increasing action of GTE on expression of PPARδ signaling pathway-related genes in the white adipose tissue. Expressions of genes relating to EGCG signaling pathway that is critical for exhibition of physiological effects of EGCG were also associated with the different effects of GTE. Here, we show that anti-obesity effect of GTE differs depending on types of fats or fatty acids that consist HF diet and could be attenuated by saturated fatty acid.

Highlights

Green tea and its major polyphenol epigallocatechin-3-O-gallate (EGCG) have suppressive effect on dietary obesity
olive oil (OO)-green tea extract (GTE) group had lower weights of perirenal fat and epididymal fat than OO group that had higher weights than Normal group (Fig. 1b,c). These results suggest that the anti-obesity effect of GTE depends on the types of fat that consists HF diet and GTE could more effectively suppress the obesity induced by OO than that that induced by beef tallow (BT)
We investigated the effect of GTE intake on obesity induced by four types of HF diets that were mainly consisted with different types of fat, OO or BT, or with different composition of fatty acids, unsaturated fatty acid (UFA) or saturated fatty acid (SFA)

Summary

Introduction

Green tea and its major polyphenol epigallocatechin-3-O-gallate (EGCG) have suppressive effect on dietary obesity It remains unsolved what type of diet on which they exhibit high or low antiobesity effect. GTE effectively suppressed obesity induced by unsaturated fatty acid-enriched HF diet with the stronger effect compared with that induced by saturated fatty acid-enriched HF diet These differences would be associated with the increasing action of GTE on expression of PPARδ signaling pathway-related genes in the white adipose tissue. We examined the effect of green tea extract (GTE) on obesity induced by the HF diet based on olive oil that contains abundant unsaturated fatty acid (UFA), especially oleic acid, and by the HF diet based on beef tallow containing high saturated fatty acid (SFA).

Methods

Results

Conclusion