Abstract
BackgroundPharmacogenetic studies are essential in understanding the interindividual variability of drug responses. DNA sample collection for genotyping is a critical step in genetic studies. A method using dried blood samples from finger-puncture, collected on DNA-cards, has been described as an alternative to the usual venepuncture technique. The purpose of this study is to evaluate the implementation of the DNA cards method in a multicentre clinical trial, and to assess the degree of investigators' satisfaction and the acceptance of the patients perceived by the investigators.MethodsBlood samples were collected on DNA-cards. The quality and quantity of DNA recovered were analyzed. Investigators were questioned regarding their general interest, previous experience, safety issues, preferences and perceived patient satisfaction.Results151 patients' blood samples were collected. Genotyping of GST polymorphisms was achieved in all samples (100%). 28 investigators completed the survey. Investigators perceived patient satisfaction as very good (60.7%) or good (39.3%), without reluctance to finger puncture. Investigators preferred this method, which was considered safer and better than the usual methods. All investigators would recommend using it in future genetic studies.ConclusionWithin the clinical trial setting, the DNA-cards method was very well accepted by investigators and patients (in perception of investigators), and was preferred to conventional methods due to its ease of use and safety.
Highlights
Pharmacogenetic studies are essential in understanding the interindividual variability of drug responses
Pharmacogenetic studies are essential both for understanding the variability of drug response observed among patients and furthering the development of personalized medicine [1,2,3]
Peripheral blood sample collection from patients is a critical step in obtaining DNA for genetic or pharmacogenetic studies
Summary
Pharmacogenetic studies are essential in understanding the interindividual variability of drug responses. Peripheral blood sample collection from patients is a critical step in obtaining DNA for genetic or pharmacogenetic studies. This may require the involvement of different centres, couriers and laboratory resources. Blood samples obtained by venepuncture (the standard DNA collection method) often cannot be used due to medical, logistical or cultural reasons. Use of such samples is feasible, but prohibitively expensive. This method is a labour-intensive way of obtaining DNA at the doctor's office and is associated with potential security problems. Logistical difficulties in managing liquid or frozen samples are associated with high costs [6, 7]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
