SAT663 Determination Of Mitochondrial DNA Mass In Women With Polycystic Ovary Syndrome

Abstract

Abstract Disclosure: M. Rojo: None. A.L. Millán: None. H. Pérez: None. C.M. Pautasso: None. P.N. Maidana: None. M.G. Emily: None. G.E. Cerrone: None. G.D. Frechtel: None. Polycystic ovary syndrome (PCOS) is characterized by the presence of obesity and/or metabolic syndrome that can influence mitochondrial DNA content. Objective: To evaluate the mitochondrial DNA mass in women with PCOS in comparison with control women and its relationship with metabolic parameters and absolute telomeric length (aTL). Materials and methods: We studied 81 women with PCOS and 57 control women aged 16 to 46 years. The copy number of mitochondrial DNA (mtDNA) was determinated in peripheral blood leukocytes by quantitative PCR in real time, SYBR Green method. The relationship between mtDNA copy number and copy number of the single copy β2 microglobulin gene was measured. Anthropometric, metabolic and hormonal variables (PCOS) were studied to carry out linear regression between the ADNmt content and the distinct comparative form variables between the distinct groups. Statistical analysis was carried out in SPSS version 25 with a significance level of 0.05. Results: Compared to controls, PCOS patients have higher body mass index (BMI), waist circumference (WC), levels of TG, LDL-c, total cholesterol, fasting plasma glucose, and lower levels of HDL-c. We observed that mitochondrial DNA mass was significantly lower in the presence of PCOS (216,40±140,9 vs 164.59 ± 102.07; p = 0.020). When corrected for age, this result is still significant. PCOS women were classified as metabolically healthy PCOS (PCOS-MH) and PCOS with metabolic syndrome (PCOS-SM). We observed a progressive decrease in mtDNA content between control, PCOS-MH, and PCOS-SM (215,42±143,39; 179,64±106,12; 149,69±93,13), with statistical significance between control and PCOS-SM (p=0,044), maintained after age correction (p=0,023). By linear regression we found association of mtDNA mass with BMI (p=0,013) and WC (p=0,030). An association tendency was observed between lower mitochondrial DNA mass and higher HOMA index (p=0,086), also, with longer aTL (p = 0.088). Conclusion PCOS-MH was defined as an intermediate phenotype between controls and PCO-SM, when mass mtDNA mass, aTL and metabolic variables were studied, reflecting the oxidative stress associated with PCOS that could cause damage mtDNA, proteins and lipids. Presentation: Saturday, June 17, 2023