SAT-334 Proinflammatory Cytokines Modulate Resistin Expression in Breast Cancer Cells

Abstract

Resistin is a proiflammatory adipokine produced by the white adipose tissue macrophages and adipocytes. Resistin expression is upregulated in breast cancer tissue and positively correlates with tumor stage, tumor size and lymph node metastasis. Previous studies found that resistin increases breast cancer invasiveness, stimulates epithelial to mesenchymal transition (EMT) and induces acquisition of cancer stem cell properties in breast cancer cells. Breast cancer cells express a variety of cytokines and their receptors, and resistin mRNA expression was previously detected in HCT-116 colorectal cancer cells. By using qRT-PCR analysis, we amplified resistin mRNA and compared its relative expression levels in a panel of breast cancer and breast non-carcinogenic cell lines, including MCF-7, T47D, BT474, HCC1806, MDA-MB-468, MDA-MB-231, and MCF-10A cells. We found that MCF-7, T47D, and HCC1806 cells express detectable levels of resistin mRNA. Using MCF-7 cells, we further investigated whether this expression can be modulated by treatment with different cytokines (resistin, TNFα, or TGFβ). Our results indicated that resistin (in concentration of 12.5 ng/ml), TNFα and TGFβ (in concentrations of 10 ng/ml) upregulated the expression of resistin mRNA in a time-dependent manner. In conclusion, we report that breast cancer cells express resistin mRNA, and that this expression is upregulated after treatment with resistin, TNFα, and TGFβ. To our best knowledge, this is the first study describing resistin mRNA expression in breast cancer cells and its alteration by proinflammatory factors. Further experiments are in progress.