SAT-210 - Regeneration linked miRNA in acute liver failure can predict clinical recompensation after DAA associated HCV cure: a novel biomarker to inform treatment selection in decompensated HCV cirrhosis?

Abstract

Cardiovascular diseases (CVD) are the major cause of morbidity and mortality in Malaysia, and dyslipidemia is a known contributing factor to the development of CVD. MicroRNAs (miRNAs) have been identified as potent post-transcriptional regulators of lipid metabolism, making it an important area of research to be explored in lipid disorders. Until today, no published data was reported on circulating miRNA among young men with dyslipidemia.To determine the differentially expressed miRNA between with dyslipidemia and nondyslipidemia in young men.Fourteen young males (32 ± 0.45 years) were recruited at UKMMC after obtaining written consent and were divided into dyslipidemia and non-dyslipidemia. Mean values for TC and LDL were significantly higher in dyslipidemia men compared to non-dyslipidemia (4.20 ± 0.8 mg/dl vs. 3.24 ± 0.33 mg/dl and 5.9 ± 0.67 mg/dl vs. 4.9 ± 0.31 mg/dl) (p<0.05), respectively. TG and HDL levels were not significant between both groups. Ten ml of blood was collected for total RNA extraction which then subjected to quantitative PCR using miRCURY LNA™ Universal RT microRNA PCR Serum/Plasma Focus Panel. All data was analysed using GenEx software (reference gene: miR-423-5p) and SPSS v.16 with p<0.05 was considered significant.Our results revealed six miRNAs were significantly upregulated and two were downregulated in dyslipidemia men compared to non-dyslipidemia (p<0.05). miR-144 is the most significant upregulated miRNA. This miRNA plays an important role in lipid metabolism as it targeted Liver X Receptor (LXR), an established key modulators of lipid metabolism. Eventually, it led to accumulation of cholesterol and attenuated cholesterol efflux by suppressing ABCA1 expression. ABCA1 is known to facilitate the efflux of cholesterol and phospholipids to lipid-poor lipoproteins.The study has successfully discovered a list of miRNA in circulating blood of young men with dyslipidemia. Further validation of these candidate miRNA is required before they are used as biomarkers for the current health problem in our community.