SARCOPLASMIC RETICULUM IN THE ADDUCTOR MUSCLES OF A BERMUDA SCALLOP: COMPARISON OF SMOOTHVERSUSCROSS-STRIATED PORTIONS

Abstract

The adductor muscle of the Bermuda scallop, Pecten ziczac, is composed of two different types of muscle: cross-striated and smooth. The major portion consists of ribbon-shaped cross-striated muscle cells averaging about ten µm by 1.5 µm in cross section. Each cell contains only one myofibril. In some of the wider cells, an extra sarcomere sometimes is inserted in the lateral part of the myofibril creating a vernier. The individual striated muscle cells do not span the entire length of the adductor but are connected at their ends via junctions similar to intercalated discs. The minor portion of the adductor muscle consists of smooth muscle cells which are fusiform averaging 6 µm in diameter. There are no specialized cell surface invaginations in either muscle type that correspond to a T-tubule or caveolae system. The sarcoplasmic reticulum in both muscles is confined to the area just beneath the cell surface. In both muscle types, the sarcoplasmic reticulum systems have distended vesicles connected to the cell membrane via surface couplings. These vesicles are interconnected by one to seven tubular elements of the sarcoplasmic reticulum to form a closed and continuous system. The tubular elements of the sarcoplasmic reticulum in the cross-striated muscle are fairly uniform in bore, 40 nm in diameter, as compared to the irregular bore in smooth muscle, 15-40 nm. The striated muscle has twice as much of its surface covered with sarcoplasmic reticulum as does the smooth muscle. Moreover, the striated muscle cells have about 4% of their cross-sectional area devoted to sarcoplasmic reticulum while the smooth muscle cells have only 0.5%. This eight-fold difference in the amount of sarcoplasmic reticulum in the striated muscle is consistent with its reported 50-fold faster contraction rate and 128-fold faster relaxation time over that of the scallop adductor smooth muscle.