Abstract
A procedure for rapid determination of nitrate in the fresh petiole sap using an ion specific electrode was developed. A highly significant correlation (R2-0.92) was obtained between the nitrate measured by the sap test and the conventional oven-dried tissue method. The effects of five nitrogen(N) rates ranging from 0 to 268 kg ha-1, and five dates of sampling dates beginning at tuber initiation, on the sap nitrate concentration were investigated. The nitrate level increased in proportion to N fertilizer rate. The nitrate level was generally higher at tuber initiation and decreased as the season progressed. The rate of decrease was related to the N supply in the soil. At N rates of 0 and 67 Kg ha-1, the average weekly decrease in the nitrate level was greater than 100 ppm. Based on yield response, the nitrate levels were partitioned as deficient adequate and excessive, and a critical nutrient range was established. The sap test offers a tactical approach for corrective in-season fertilization and a means to increase the efficiency of both fertilizer and available soil N.
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