Abstract
Amaranthus tricolor is a potential accumulator of nitrate but nutrient sufficiency guidelines have not been developed for this vegetable. We investigated whether the shoot stem of A. tricolor is a suitable index for crop nitrate status, and whether freezing the stem, which aids sap extraction, would render it unsuitable for the analysis of nitrate and other nutrients. We grew A. tricolor at two nitrogen (N) rates (100 and 200 mg L−1 as nitrate-N) and established that sap nitrate from both fresh and frozen-thawed stems did not differ but was significantly increased by additional N fertilizer. Shoot growth was correspondingly higher with additional N. Therefore, the stem is a suitable index for crop nitrate. Boron (B), calcium (Ca), iron (Fe), magnesium (Mg), manganese (Mn), molybdenum (Mo), sodium (Na) and sulfur (S) were significantly affected by frozen storage but potassium (K), phosphorus (P) and zinc (Zn) were not. A single sufficiency guideline is possible for these nutrients, for use with both fresh and frozen-thawed stem sap.
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