Abstract
Staphylococcus aureus is the most common cause of surgical site infections and its treatment is challenging due to the emergence of multi-drug resistant strains such as methicillin-resistant S. aureus (MRSA). Natural berry-derived compounds have shown antimicrobial potential, e.g., ellagitannins such as sanguiin H-6 and lambertianin C, the main phenolic compounds in Rubus seeds, have shown antimicrobial activity. The aim of this study was to evaluate the effect of sanguiin H-6 and lambertianin C fractionated from cloudberry seeds, on the MRSA growth, and as treatment of a MRSA biofilm development in different growth media in vitro and in vivo by using a murine wound infection model where sanguiin H-6 and lambertianin C were used to prevent the MRSA infection. Sanguiin H-6 and lambertianin C inhibited the in vitro biofilm development and growth of MRSA. Furthermore, sanguiin H-6 showed significant anti-MRSA effect in the in vivo wound model. Our study shows the possible use of sanguiin H-6 as a preventive measure in surgical sites to avoid postoperative infections, whilst lambertianin C showed no anti-MRSA activity.
Highlights
The recent increase in the number and complexity of surgical interventions increases the number of surgical site infections (SSI) [1,2]
The Minimum inhibitory concentrations (MIC) of the mixture of sanguiin H-6 and lambertianin C was not significantly different compared to each compound separately (Figure 2C)
The MIC of the mixture of sanguiin H-6 and lambertianin C w significantly different compared to each compound separately (Figure 2C)
Summary
The recent increase in the number and complexity of surgical interventions increases the number of surgical site infections (SSI) [1,2]. The SSIs are the most common healthcareassociated infections and are associated with prolonged antibiotic therapy, longer postoperative hospital stays, additional surgical procedures, even with treatment in intensive care units and higher mortality [3]. Staphylococcus aureus is the most common pathogen causing SSIs [4]. Methicillinresistant S. aureus (MRSA) is isolated in a high percentage of SSIs [4,5,6]. MRSA infections oacpwaracabtreaarnrntaaehhseeuucttdaiieietssstmemmerreemtMhSnresaddiooititnceagai[brrbrcnt1pabeehaoayr1thSnyreocbss]ey[.mdrtb,iee9elaameiovv,hherual1nhcieetaeerl0taochirrevrtag]careeihue.elacchestasiuiresaahecltgeisd(lninrmsMialcndltdaeiihiaeannu-nnRnnserrrrec-aeeSeatecesdanAqlueqpe[stcs9usnuhrre)oii,oessitdi1isirirdtissc0tveuteeasiui]iectnnv.rsuhuct[eoSfeie8pepoval.d]acreSa.mttpottuo.muosesrorMudnatoefiafsssuuatitotivcevrrnts[ereeciece8sexputlao]tyioddst.ramvhivaanabieMisienymydgintrlsmcroshi,soaratrlyearnS[lipoeo7enatSoenr]onspsbI,vrigirsgxacenwe[eeectc7grarhahrono,]rnom,itahemhScadgnowhSoigbpendsIseaiihnlpsgponermioietfiiccmcattefrhaaalarebmSoleunlraieiSreizyszs-encbIsaireasonciieSrtstsomrei[gli.toeao4sfsaaiuaiin–tnSlnnsuesm6fStcgfdreca]ceIee-s.oonsrurmiMmimttenssn[olt4oufapRgspoe]rgftta.aScefreoalerrtaAydnfeMierriocttidnedihioninemsnsnepgitts,tffsohoieepeactwiopcacmractttofntthhthiiiiliobotoodoeletniinhnsnrestaneegoseesstl[12,13M].icTrohbeiraelfroersei,statoncceoins turnoflotrhtuenaspterleyaidncorefasainntgibainodticmarensyisSta. anucreeuasnsdtratirnesa,tammoicnrgoobtihalienrfse[c1t1io],nhsaivnecgluadininedg rSeSdIuictedissnuescceepsstaibriylittyo oirncvoemstpilnetreerseesaisrtcahncaendto dgelyvceolpopepmtiednets o[1f2n,1e3w]. Tphlaenbt aecxtterraicctisdathl antohna-avnetisbhioowticednaatnutria-Ml pRroSdAupctrsocpaenrtbieesfpoeurnsde.inSov,afroioruinssstoaunrccee,sthinisclaubdiilnitgy phalasntbse, einnsercetcse,natnlyimdaelss,carinbdedfuinngie. xTthraecret aorfe daneseenrtortmhooruns n(Luymcibuemr osfhpawlaini)t, eaxntrdacItnsdthiaant ghoaovseesbheorwrye(dPhaynltlia-nMthRuSsAempbrloicpae),rtpieasmppe(rAsreb.uStou,sfpoarvianrsiit)aonrcem, athnigsoagbiinligtyerh(aCsubrecuenmareacmenadtlay) [d1e4s–c1r7ib].ed in extract of desert thorn (Lycium shawii), and Indian gooseberry (Phyllanthus embliNcaa),tupraaml pb(eArrrbieustusppoasvsaersisi) oprhmeannoglioc gicnogmerpo(Cuunrdcus mtahaamt adhaa)v[1e4–s1h7o].wn promising antimNicartoubraial lbearnrdiesapnotis-sbeisosfiplmheneoffleicctcsomagpaoiunsntdsMthRaStAha[v1e8]s.hAowmnopnrgomthiessinegbaenrtriym-dicerroibvieadl caonmd paonutin-bdiso, fiellmlageiftfaencntsinasgaairnestpMarRticSuAla[r1ly8].inAtemreostninggt.hTesheeybearrrey-dmeorlievceudlecsomthpaot uhnadvse, sehlloagwitnananntiinms iacrreopbaiartliaccutliavriltyyiangtearienssttinfugn. gTih,evyiraurseems,oalnedcubleascttehraiat ,hianvcelusdhionwgnMaRntSiAmi[c1r9o,b2i0a]l
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