Sandwich-Type Electrochemical Aptasensor for Highly Sensitive and Selective Detection of Pseudomonas Aeruginosa Bacteria Using a Dual Signal Amplification Strategy

Abstract

An electrochemical aptasensor developed to realize the detection of Pseudomonas aeruginosa (P. aeruginosa) bacteria based on a signal amplification strategy. The carbon screen-printed electrode (CSPE) surface was modified by MIL-101(Cr)/Multi-walled carbon nanotubes (MWCNT), which significantly increased the effective surface area of the electrode, thus resulting in further F23 aptamer immobilization at the surface of the modified electrode. As a result, the P. aeruginosa can be efficiently captured onto the surface of the aptasensor. Moreover, aptamer immobilized on the two-dimensional graphitic carbon nitride complex with silver nanoparticles (AgNPs/c-g-C3N4/Apt) was used as an electrochemical signal label, connected to P. aeruginosa bacteria at the modified electrode. This strategy increased the aptamer surface density and the sensitivity for detecting P. aeruginosa. Also, the resultant material was thoroughly characterized using Fourier transform infrared (FT-IR), X-ray diffraction (XRD), thermogravimetric analysis (TGA), field emission scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM), and Brunauer-Emmett-Teller (BET) analysis techniques. A highly sensitive voltammetric aptasensor for P. aeruginosa detection was obtained via this strategy at the limit of detection of 1 Colony-forming unit (CFU)/mL (σ = 3). Therefore, this proposed strategy with dual signal amplification can be a promising platform for simple, practical, reliable, and sensitive method for P. aeruginosa.