Sıçan Primer Nöron Kültüründe Agmatinin Doz-Süre-Etki Profili

Abstract

Agmatine is a newly found neurotransmitter and its role in important modulations of central nervous systems how the pharmacological importance of agmatine. The aim of this study was to investigate the possible toxic and proliferative effects of agmatine, which is known to have endogenous neuroprotective effect, on primary rat neuron culture. In this context, it has been tried to determine the possible toxic and protective effects of agmatine at 8 different exposure times at 5 different doses. Neuron cultures obtained from brain cortex in the neonatal rats are treated with agmatine at 15 min, 30 min, 1 h, 2 hours, 4 hours, 8 hours, 12 hours, 24 hours, doses of 10-2, 10-3, 10-4, 10-5 and 10-6 M. Then, the dose duration effect relationship was determined with MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) proliferation kit. According to the results of the viability assay, a statistically significant neuroprotective effect was observed in 10-5 and 10-6 M doses, especially in 1 hour and 24 hours exposure periods. In addition, proliferation was observed only at a concentration of 10-4 M for 24 hours. However, no statistically significant difference in toxic effect was observed. Consequently agmatine showed neuroprotective effect in the primary rat neuron culture, especially at the lowest dose and the longest exposure.