Abstract
The mean endothelial cell area was estimated from specular photomicrographs of 6 normal and 8 low cell density corneas employing two different sampling procedures: that of a fixed and that of a variable frame analysis. Attention is drawn to the fact that these two methods differ with respect to their sampling rules: with the fixed frame method, which cells to include in the sample are defined by the frames, while the variable frame does not employ any sampling rules other than, perhaps, to include as many cells as possible. The sampling corresponding to a variable frame method is shown to result in an underestimation of the true mean cell area especially for small samples. This result is attributed to the fact that when no fixed frame defines the sampling area, the effective sampling area becomes that of within a given area, whereby large cells have a higher probability of being excluded from the sample than small cells. A correct estimate of the mean cell area could be obtained when the sampling was that of a fixed (rectangular) frame properly corrected for the edge effect.
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