Abstract
A 5 min equilibration of 0.250 mL serum sample with room air containing 5.6% CO2, by placing the gas tube < 1 cm above the sample-surface, enables a standardized determination of calcium ion concentration (cCa2+), referred to pH 7.40 and 37 °C, in aerobically handled specimens. Using this method, standardized measurement of calcium ions in serum is possible with a bias less than 2%, even in samples with pH-changes acquired during extended storage.
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