Abstract
A modified sample clean-up method for the HPLC of catecholamines (CA) in biological samples was proposed. At the adsorption procedure the loss of alumina was neglected by placing a disk filter on the bottom disk in the Mini column, and also a top filter on the surface of the sample solution. Plasma and heart extract were adsorbed onto alumina in Sepacol Mini tube. In order to obtain a constant and efficient adsorption of CA, the authors introduced the improved rotary mixer for the rotation of alumina column. By mixing the CA sample solution and alumina with rotary mixer, the recovery and reproducibility of CA were considerably improved comparing with the conventional method. HPLC with the clean-up procedure was applied to the determination of CA in human plasma and rat heart. The utility of the proposed clean-up method of CA (norepinephrine, epinephrine and dopamine) was sufficiently recognized.
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