Abstract
Antibiotic resistance is a growing concern in the treatment of infectious disease worldwide. Point-of-care (PoC) assays which rapidly identify antibiotic resistance in a sample will allow for immediate targeted therapy which improves patient outcomes and helps maintain the effectiveness of current antibiotic stockpiles. Electrochemical assays offer many benefits, but translation from a benchtop measurement system to low-cost portable electrodes can be challenging. Using electrochemical and physical techniques, this study examines how different electrode surfaces and bio-recognition elements, i.e. the self-assembled monolayer (SAM), affect the performance of a biosensor measuring the hybridisation of a probe for antibiotic resistance to a target gene sequence in solution. We evaluate several commercially available electrodes which could be suitable for PoC testing with different SAM layers and show that electrode selection also plays an important role in overall biosensor performance.
Highlights
IntroductionDNA and antibody-based sensors are popular choices for analyte detection, taking advantage of their specific interactions to accurately identify the presence of a target in a sample
The molecular detection of biomolecules through the application of electrochemical techniques has been widely studied due to the high sensitivity, specificity and simplicity of these methods [1].DNA and antibody-based sensors are popular choices for analyte detection, taking advantage of their specific interactions to accurately identify the presence of a target in a sample
A number of different electrodes were examined by microscopy and electrochemical characterisation in order to assess their suitability as a platform for point-of-care DNA biosensing
Summary
DNA and antibody-based sensors are popular choices for analyte detection, taking advantage of their specific interactions to accurately identify the presence of a target in a sample Both DNA and protein detection can be performed through the inclusion of redox reporters, such as DNA intercalators or electroactive enzyme substrates [2,3], through the labelling of the target or capture molecules [4,5], or through direct detection of the target’s effect on the interfacial properties of the electrode when under electrochemical measurement [6,7]. Modern diagnostics are tending towards point-of-care (PoC) tests: assays which can be performed at the bedside, in the field or at home Such tests are often expected to provide results in minutes, unlike the centralised laboratory tests which may take hours to complete or require the collection of large sample numbers to be viable. PoC tests typically use disposable test cartridges or strips which limit opportunities for cross-contamination and minimise the amount of training and interaction
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
