Salvianolic acidB inhibits myofibroblast differentiation and extracellular matrix accumulation in nasal polyp fibroblasts via the TGF-β1 signaling pathway.

Abstract

In the process of nasal tissue remodeling, nasal fibroblasts serve an important role via myofibroblast differentiation and the production of extracellular matrix(ECM). Nasal fibroblast abnormalities can lead to conditions such as chronic rhinosinusitis. Salvianolic acidB (SalB), a water-soluble active pharmaceutical compound extract from the root of the traditional Chinese medicine Salviamiltiorrhiza, displays antioxidative, antiproliferative and antifibrosis properties. The present study aimed to investigate the mechanism underlying the effects of SalB on nasal polyp fibroblast (NPF) myofibroblast differentiation and ECM accumulation. Primary NPFs were obtained from nasal polyps of patients with chronic sinusitis. The proliferative and cytotoxic effects of SalB on NPFs were evaluated by performing the Cell Counting Kit-8 assay. The Transwell assay was conducted to assess cell migration. α-smooth muscle actin (α-SMA), TGF-β1 receptor(TβR)-I, TβR-II, Smad2/3 mRNA and protein expression levels and (p)-Smad2/3 phosphorylation levels were measured via reverse transcription-quantitative PCR and western blotting, respectively. TypeIII collagen and fibronectin levels were analyzed by ELISA. The results indicated that SalB significantly downregulated TGF-β1-induced α-SMA, fibronectin and collagenIII expression levels in NPFs. Similarly, SalB significantly decreased TGF-β1-induced TβR-I, TβR-II, p-Smad2/3, MMP-2 and MMP-9 mRNA and protein expression levels in NPFs. Furthermore, SalB significantly decreased TGF-β1-induced NPF migration. Therefore, the present study indicated that SalB inhibited myofibroblast differentiation and ECM accumulation in nasal fibroblasts, suggesting that SalB may inhibit nasal polyp formation via certain mechanisms.