Abstract
Previous studies have found that Salvia miltiorrhiza (SM) injection have a protective effect on the iron overloaded (IO) heart. However, the mechanisms are not completely known. In the present study, we investigated the underlying mechanisms based on the iron transport-related proteins. The rats were randomly divided into five groups: control, IO group, low-dose SM group, high-dose SM group, and deferoxamine control group. Iron dextran was injected to establish the IO model. After 14 days of treatment, cardiac histological changes were observed by hematoxylin and eosin (H&E) staining. Iron uptake-related proteins divalent metal transporter-1 (DMT-1), transferrin receptor-1 (TfR-1), and iron export-related proteins ferroportin1 (FP1) in the heart were detected by Western blotting. The results showed that SM injection decreased cardiac iron deposition, ameliorated cardiac function, and inhibited cardiac oxidation. Most important of all, SM injection downregulated the expression of DMT-1 and TfR-1 and upregulated FP1 protein levels compared with the IO group. Our results indicated that reducing cardiac iron uptake and increasing iron excretion may be one of the important mechanisms of SM injection reducing cardiac iron deposition and improving cardiac function under the conditions of IO.
Highlights
Iron is vital to the maintenance of normal cellular structure, function, and metabolic activity
Contents Analysis of Salvia miltiorrhiza (SM) Injection. e three main constituent of SM injection was detected by high performance liquid chromatography with ultraviolet (HPLC-UV) shown in Figure 1. e actual concentrations of the salvianolic acid A is 2.15 mg/mL, protocatechuic aldehyde is 0.44 mg/mL, and salvianolic acid B is 1.01 mg/mL
Salvia miltiorrhiza (SM), known as Danshen, is a traditional Chinese medicine used for inducing blood circulation and removing blood stasis, and it has been used for the Prussian blue staining (a) Prussian blue staining
Summary
Iron is vital to the maintenance of normal cellular structure, function, and metabolic activity. E heart is one of the main target organs for iron deposition under conditions of IO. Cardiomyocytes maintain cardiac iron homeostasis by regulating iron uptake/ storage in order to maintain normal cardiac function. Ferroportin 1 (FP1), which is expressed in the heart, liver, and other tissues in humans and mice, has been regarded as exporter of cellular iron [7]. Based on these previous findings, this study investigated the regulative effects of Salvia miltiorrhiza
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