Abstract
The separation of hydroxybenzoic acid isomers by salting-out chromatography using Amberlite CG-50 (Na + form) as stationary phase and acidic sodium chloride solution as mobile phase has been studied. The distribution coefficients of hydroxybenzoic acids increased with increase in the concentration of sodium chloride and with decrease in the pH of the solution. The best separation was achieved when a mixture of the o- and m-isomers or the o- and p-isomers was eluted at 40 ± 1° through a column of length 150 mm and I.D. 10 mm with 2 M sodium chloride solution of pH 1.8. The determination of the acids in the eluates by UV spectrophotometry proved that the recovery of the acids was almost quantitative.
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