Salt tolerance mechanism of a hydrocarbon-degrading strain: Salt tolerance mediated by accumulated betaine in cells

Abstract

Rhodococcus sp. HX-2 could degrade diesel oil in the presence of 1%–10 % NaCl. The compatible solute betaine accumulated in cells with increasing NaCl concentration, and this was found to be the main mechanism of resistance of HX-2 to high salt concentration. Exogenously added betaine can be transported into cells, which improved cell growth and the percentage degradation of diesel oil in the presence of high [NaCl] in solution and in soil. Scanning electron microscopy data suggested that addition of exogenous betaine facilitated salt tolerance by stimulating exopolysaccharide production. Fourier-transform infrared analysis suggested that surface hydroxyl, amide and phosphate groups may be related to tolerance of high-salt environments. Four betaine transporter-encoding genes (H0, H1, H3, H5) and the betaine producer gene betB were induced in Rhodococcus sp. HX-2 by NaCl stress. The maximal induction of H0, H1, H3 and H5 transcription depended on high salinity plus the presence of betaine. These results demonstrate that salt tolerance is mediated by accumulated betaine in Rhodococcus sp. HX-2 cells, and the potential of this strain for application in bioremediation of hydrocarbon pollution in saline environments.