Abstract
The aim of the project was to find new catalysts capable of chlorolactone biotransformation. Three bicyclic chlorolactones with structures possessing one or two methyl groups in their cyclohexane ring were subjected to screening biotransformation using seven bacterial strains and one fungal strain from a salt mine. Three strains of bacteria (Micrococcus luteus Pb10, Micrococcus luteus WSP45, Gordonia alkanivorans Pd25) and one fungal strain (Aspergillus sydowii KGJ10) were able to catalyse hydrolytic dehalogenation of one substrate. The classification of the strains that were effective biocatalysts was confirmed by 16S rDNA analysis. The best result (76%) was obtained using Aspergillus sydowii KGJ10. All strains catalysed hydrolytic dehalogenation without changing the conformation. The equatorial position of the chlorine atom in the substrate turned out to be warrant of the positive result of the biotransformation process.
Highlights
Halogenated organic compounds constitute one of the largest groups of environmental chemicals [1]
Three bicyclic chlorolactones with the gem-dimethylcyclohexane ring (1) and the methylcyclohexane ring (2 and 3) were used as substrates for biotransformation by microorganisms isolated from bioaerosol emanating from the salt mine (Salt Mine KGHM "Polkowice-Sieroszowice")
Seven bacterial strains: Aerococcus viridans WSP451, Gordonia alkanivorans Pd25, Micrococcus luteus, and one strain of a filamentous fungus Aspergillus sydowii KGJ10 were used in biotransformation
Summary
Halogenated organic compounds constitute one of the largest groups of environmental chemicals [1]. Over 4000 halogenated compounds have been identified to date, in almost all classes of organic chemicals [2]. Neomangicol A and B have demonstrated in vitro cytotoxic activity toward the HCT-116 human colon tumor cell line [2]. Another example of a halogen containing drug is metoclopramide, a dopamine receptor antagonist used in the treatment of vomiting and nausea [4]. Many agricultural products such as herbicides, fungicides and insecticides are halogenated compounds [5,6]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
