Abstract
We report on a salmonellosis-outbreak due to Salmonella Enteritidis phage type 14b resistant to nalidixic acid (S. Enteritidis PT14b Nx) among residents and employees of a student residence in Austria, September 2010. The outbreak was described and analysed by a retrospective cohort study, and microbiological environmental investigations were conducted to identify the outbreak source(s) and the reservoir of the outbreak strain. A total of 66 persons fulfilled the outbreak case definition including 14 laboratory-confirmed cases. Food specific cohort-analyses by day revealed that consumption of potato salad (RR: 1.65, 95%CI: 1.35–2.01, p=0.001) and a cheese-sausage cold plate (RR: 2.24, 95%CI: 1.29–3.88, p=0.002) on 14 September was associated with being an outbreak case. We hypothesised that cross-contamination with S. Enteritidis PT14b Nx positive eggs had occurred during preparation of the potato salad and cold plate as a result of preparing in parallel egg-containing breaded cutlets on 14 September. A traced laying hen holding in eastern Austria was identified as the sole source of the consumable eggs in the student residence. By applying the legally mandated sampling method for epidemiological-related laying hen farms (one pooled dust sample à 150g, two paired boot swabs cultured separately), the outbreak strain could not be detected. Our findings, that legally required sampling methods for laying hen farms failed to detect the causative pathogen in a laying hen holding, despite an epidemiological link, underline the request stated by the European Food Safety Authority Panel on Biological Hazards for a more sensitive sampling plan in epidemiologically-associated laying hen flocks.
Highlights
In the European Union (EU), food-borne outbreaks are mandatorily reported since 2003 [1]
We report on a salmonellosis-outbreak due to Salmonella Enteritidis phage type 14b resistant to nalidixic acid
Food specific cohort-analyses by day revealed that consumption of potato salad (RR: 1.65, 95%CI: 1.35–2.01, p=0.001) and a cheese-sausage cold plate (RR: 2.24, 95%CI: 1.29–3.88, p=0.002) on September was associated with being an outbreak case
Summary
In the European Union (EU), food-borne outbreaks are mandatorily reported since 2003 [1]. Between 30% and 60% of the reported outbreaks have been caused by Salmonella [2]. 1,255 Salmonella outbreaks reported within the past five years, the most frequent serovar was Salmonella. Enteritidis, accounting for more than 82% (unpublished data). Eggs from Salmonella-positive laying hen flocks and products made from such eggs were the most frequently associated food vehicles [2]. Enteritidis phage type (PT) 14b was considered a rare causative agent of human salmonellosis in the EU [3]. In 2001, Norway, Sweden and Finland reported increased numbers of cases of infection with
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