Abstract
The statistical technique of response surface methodology was used to optimize the component concentrations of a Salmonella lysine medium for conductance responses on a Malthus microbial growth analyser. When the original and modified media were compared, conductance changes in pure cultures improved by 135 μS on average, and those from fopds both naturally contaminated and spiked with salmonellas by 220 μS. Salmonellas were successfully isolated from all assays after subculturing conductance broths onto xylose lysine desoxycholate agar and confirming by serology.
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