Sağlıklı Bireylerden ve Candida – İlişkili Protez Stomatitili Hastalardan İzole Edilmiş Stok C.albicans Suşları Arasındaki C.dubliniensis Prevalansının Moleküler Yöntemlerle Retrospektif Olarak Araştırılması

Abstract

Objectives: Candida-related denture stomatitis (C-RDS), is the most frequent form of oral candidiasis, being detected in approximately 50%-65% of denture-wearing patients. Candida albicans has been shown to be the principal species responsible for inflammatory pathology. Candida dubliniensis, first described in Dublin in 1995, was found to be very similar to C. albicans in terms of phenotypic features and the type of lesion it causes. It was necessary to determine the historical prevalence of C.dubliniensis due to the possibility that the strains identified as C.albicans in the past years were C.dubliniensis. Materials and Methods: In this study, 81 yeast strains isolated from oral samples and identified as C.albicans only by photypic methods and kept in the stock collection until 2003 were re-examined by molecular methods. Results: In the genotypic examination performed by polymerase chain reaction and restriction endonuclease reactions, 4 (8.8%) of 45 C.albicans strains isolated from healthy individuals were found to be C.dubliniensis, but all 36 strains isolated from patients with C-RDS were C.albicans. Conclusions: As a result of this retrospective study, it was found that the prevalence of C.dubliniensis among 81 yeast strains isolated orally was 4.9%, but it was not effective in the pathogenesis of C-RDS.