Abstract
An organotypic tissue culture model canmaintain the cellular and molecular interactions, as well as the extracellular components of a tissue ex vivo. Thus, this 3D modelbiologically mimics in vivo conditions betterthan commonly used 2D culture in vitro models. Here, we provide a detailed workflow for generating live 3Dorganotypic tissue slices from patient-derived freshly resected salivaryglandular tissues. We also cover the processing of these tissues for various downstream applications like live-dead viability/cytotoxicity assay, FFPE sectioning and immunostaining, and RNA and protein extraction with a focus on the salivary gland radiation injury model. These procedures can be applied extensively to various solid organs and used for disease modeling for cancer research, radiation biology, and regenerative medicine.
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