Salivary malondialdehyde levels in clinically healthy and periodontal diseased individuals

Abstract

Lipid peroxidation (LPO) has been implicated in the pathogenesis of several pathologic disorders, including periodontal disease. Malondialdehyde (MDA) is one of many low molecular weight end products of LPO. This study was conducted to evaluate salivary MDA levels in generalized chronic periodontitis (GCP) subjects. The MDA levels were measured in the saliva of 104 subjects, aged 18-65 years. Three groups with different degrees of severity of GCP were established: 30 early (group 1), 30 moderate (group 2) and 14 severe (group 3). Thirty individuals (aged 25-29 years) with clinically healthy periodontium were served as control. Unstimulated whole saliva samples from study subjects were collected, centrifuged at 3000 g for 15 min and were then stored at -70 degrees C until analysed. The MDA level was determined with 2-thiobarbituric acid by a colorimetric method at 532 nm. A significant increase in the MDA level existed in the samples obtained from the three groups of patients compared to the control subjects. Increased MDA levels are with closely associated with the severity and patients status of periodontal disease that has not been previously reported. The detection of salivary MDA level may provide additional advantages in elucidating the pathogenesis of periodontal disease.