Salivary fluoride bioavailability after use of high-fluoride dentifrices with different compositions: A short-term randomized clinical trial.

Abstract

The objective of the study was to evaluate the kinetics of salivary F bioavailability after the use of high-fluoride dentifrices with different compositions and their amount of total soluble fluoride (TSF). A short-term clinical randomized trial was performed in which 15 adult participants were randomly allocated into three groups: 5000 ppm F-dentifrice, 5000 ppm F-dentifrice + TCP (tri-calcium phosphate) and 1450 ppm F-dentifrice. Unstimulated saliva was collected at different times: baseline (before toothbrushing), immediately after brushing/water rinsing and at 5, 15 and 30min and 1, 2, 4, 8 and 12h after brushing. The TSF in dentifrices and saliva samples was analysed using an ion-specific electrode. For statistical analysis, the paired t-test and Kruskal-Wallis were used with Dunn's post-test with a 95% confidence interval. There was no significant difference between the declared TSF and that found in 5000 ppm F-dentifrice and 1450 ppm F-dentifrice (p≥ 0.13); however, in the 5000 ppm F-dentifrice + TCP, approximately 500 ppm less TSF was observed (p=0.0024). The area under the curve (AUC, μg F/ml min-1 ) of both high-fluoride dentifrices (321.7 ± 84.0 and 223.6 ± 55.1 for the one without and with TCP, respectively) was higher than the conventional one (89.97 ± 15.6) attesting a higher F-bioavailability (p=0.04). Furthermore, they were able to provide F-salivary levels higher than the baseline for up to 2 h, while this time was 1 h for the 1450 ppm F-dentifrice (p ≤ 0.003). Both high-fluoride dentifrices similarly increased the salivary-F bioavailability in comparison with 1450 ppm F-dentifrice, despite the lower TSF presented by the dentifrice containing TCP.