Abstract
BackgroundMany metabolic changes develop in patients with chronic kidney disease which often necessitate frequent biochemical analysis of blood. Saliva analysis as an alternative to blood has many advantages. The aims of this study were to evaluate levels of salivary creatinine and urea in patients with chronic kidney disease in comparison to healthy individuals; to determine correlation between salivary creatinine/urea and blood creatinine/urea and to evaluate the diagnostic potential of saliva.MethodsA case control study, involving 50 patients with late stage chronic kidney disease and 49 healthy individuals as control. Blood and saliva samples were analyzed for urea and creatinine levels. Data are presented as median with interquartile range and compared using Independent Samples Mann Whitney U test. Correlation between plasma and salivary creatinine as well as urea was determined using Spearman’s correlation test. Receiver operating characteristics (ROC) analysis was done to determine the diagnostic ability of salivary creatinine and urea and cut-off values were established.ResultsMedian salivary creatinine levels were 2.60 mg/dl and 0.20 mg/dl while median salivary urea levels were 92.00 mg/dl and 20.50 mg/dl in patients with chronic kidney disease and controls respectively. Salivary levels of creatinine and urea were significantly elevated in chronic kidney disease patients (p < 0.001). In addition, there was positive correlation between blood and salivary creatinine as well as urea levels. Total areas under the curve for salivary creatinine and urea were 0.97 and 0.89 respectively. Cut-off values for salivary creatinine and urea were 0.55 mg/dl and 27.50 mg/dl respectively which gave sensitivity and specificity of 94 % and 85 % for creatinine; as well as 86 % and 93 % for urea.ConclusionsFindings of this study suggest that analysis of salivary creatinine and urea in patients with chronic kidney disease reflects their levels in blood. Hence, salivary creatinine and urea could be used as diagnostic biomarkers of chronic kidney disease.
Highlights
Many metabolic changes develop in patients with chronic kidney disease which often necessitate frequent biochemical analysis of blood
As expected the median plasma creatinine and urea were significantly higher in patients with chronic kidney disease (CKD) (p < 0.001)
Linear regression analysis performed to estimate plasma creatinine as well as urea levels in saliva showed the equations indicated in Figs. 1 and 2
Summary
Many metabolic changes develop in patients with chronic kidney disease which often necessitate frequent biochemical analysis of blood. The aims of this study were to evaluate levels of salivary creatinine and urea in patients with chronic kidney disease in comparison to healthy individuals; to determine correlation between salivary creatinine/urea and blood creatinine/urea and to evaluate the diagnostic potential of saliva. Worldwide, increasing numbers of patients are affected by chronic kidney disease (CKD) [1, 2]. Analysis of salivary creatinine and urea in patients with CKD offers many advantages that have been attributed to the use of saliva as a diagnostic fluid. A non-invasive diagnostic test with minimal risk with ability to provide a dependable evaluation of disease condition would be of worth to both the health professionals and the patients
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