Abstract
Saliva crystallization was previously studied in both humans and animals with various results. The study aimed to confirm of the presence of saliva crystallization in female Bornean orangutans (Pongo pygmaeus), to evaluate the quality of samples which were collected from animals and processed by keepers, and to test preliminarily if the saliva crystallization could be connected with menstrual cycle and could serve as a cheap, quick and simple method for the basic monitoring of their reproductive status. The research was carried out from September 2014 to January 2015. Sampling of saliva was done in three female orangutans from three zoological gardens (Dvur Kralove, Usti nad Labem, Bojnice) daily, mostly by tongue prints on glass slides with ground edges or by sampling directly from the mouth using plastic spoons from which the saliva was transferred onto glass slides. Samples were evaluated by light microscopy with ×400 magnification. The quality of the sample and type of crystallization was assessed for two different approaches. In total, 246 samples were evaluated. We confirmed the presence of saliva crystallization in orangutans. The quality of samples was variable however acceptable. Unfortunately, it was impossible to detect exact fertile period in two females. However in one orangutan female, when the crystallization was evaluated by the approach typically used in humans, we discovered that saliva crystallization during the fertile period significantly differed from saliva crystallization in the non-fertile period. This points out the possibility of using saliva crystallization for detection of the fertile period in orangutans. However, further research was recommended.
Highlights
Bornean orangutans (Pongo pygmaeus) are considered an endangered species with a decreasing population trend [1]
The aims of this study were 1) to prove the existence of saliva crystallization in female Bornean orangutans; 2) to evaluate the quality of samples which were collected from animals and processed by keepers; 3) to test preliminarily if the saliva crystallization could be connected with menstrual cycle and serve in the future as a cheap, quick and simple method for basic monitoring of menstrual cycle in great apes
We confirmed the presence of saliva crystallization in orangutans
Summary
Bornean orangutans (Pongo pygmaeus) are considered an endangered species with a decreasing population trend [1]. Their reproduction is the slowest among great apes [2, 3]. In ex situ conservation programs, which mainly take place in zoos, there is an increasing need for proper reproduction management, including methods of ovulation and PLOS ONE | DOI:10.1371/journal.pone.0159960. Male and female orangutans are kept separately and put together for mating purposes only. In these situations, information about the fertile days of females is necessary
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