Salinity tolerance level of rice mutants (M3) derived from Ciherang and Inpari 13 varieties at seedling phase

Abstract

Salinity negatively affects rice production, therefore tolerant varieties are required to plant in an area with high salinity salinity. This especially will inhibit growth and trigger death at the seedling phase. The objective of this research is to obtain salinity tolerant rice mutants, from previously developed line by gamma ray irradiation and in-vitro selection, through a hydroponictesting in a green house. Plant materials used were third generation (M3) of rice mutants derived from Ciherang and Inpari 13 (each 45 lines), Ciherang and Inpari 13 as control parents, and Pokkali and IR20 as tolerant and sensitive salinity control, respectively. The experiment was conducted using methods developed by IRRI. This study was arranged in a completely randomized factorial design. The firstfactorwas twoconcentrationofNaCl (0 and 150 mM) and the second factor was the plant Rice varieties. The result of the study revealed that tolerant lines had higher plant height and root length than susceptible lines. This research yielded 64 rice mutants lines tolerant to salinity, i.e. 23 mutant lines of Ciherang and 45 mutant lines of Inpari 13.