Salicylic acid signal transduction: the initiation of biosynthesis, perception and transcriptional reprogramming.

Carolin Seyfferth,Kenichi Tsuda

doi:10.3389/fpls.2014.00697

Abstract

The phytohormone salicylic acid (SA) is a small phenolic compound that regulates diverse physiological processes, in particular plant resistance against pathogens. Understanding SA-mediated signaling has been a major focus of plant research. Pathogen-induced SA is mainly synthesized via the isochorismate pathway in chloroplasts, with ICS1 (ISOCHORISMATE SYNTHASE 1) being a critical enzyme. Calcium signaling regulates activities of a subset of transcription factors thereby activating nuclear ICS1 expression. The produced SA triggers extensive transcriptional reprogramming in which NPR1 (NON-EXPRESSOR of PATHOGENESIS-RELATED GENES 1) functions as the central coactivator of TGA transcription factors. Recently, two alternative but not exclusive models for SA perception mechanisms were proposed. The first model is that NPR1 homologs, NPR3 and NPR4, perceive SA thereby regulating NPR1 protein accumulation. The second model describes that NPR1 itself perceives SA, triggering an NPR1 conformational change thereby activating SA-mediated transcription. Besides the direct SA binding, NPR1 is also regulated by SA-mediated redox changes and phosphorylation. Emerging evidence show that pathogen virulence effectors target SA signaling, further strengthening the importance of SA-mediated immunity.

Highlights

The phytohormone salicylic acid (SA) is a small phenolic compound that functions as an important signaling molecule during plant immunity (Vlot et al, 2009; Robert-Seilaniantz et al, 2011; Pieterse et al, 2012)
We summarize SA signal transduction from regulation of biosynthesis, perception, to transcriptional reprogramming during plant immunity
Genetic analysis reveal that TGA2, TGA5, and TGA6 repress PR1 transcription in the absence of SA, but on the other hand are required for SA-MEDIATED TRANSCRIPTIONAL REPROGRAMMING NPR1 controls expression of more than 95% of the responsive genes to the SA-analog benzothiadiazole (BTH; Wang et al, 2006)

Summary

Carolin Seyfferth and Kenichi Tsuda*

The phytohormone salicylic acid (SA) is a small phenolic compound that regulates diverse physiological processes, in particular plant resistance against pathogens. Understanding SA-mediated signaling has been a major focus of plant research. Calcium signaling regulates activities of a subset of transcription factors thereby activating nuclear ICS1 expression. The produced SA triggers extensive transcriptional reprogramming in which NPR1 (NONEXPRESSOR of PATHOGENESIS-RELATED GENES 1) functions as the central coactivator of TGA transcription factors. The first model is that NPR1 homologs, NPR3 and NPR4, perceive SA thereby regulating NPR1 protein accumulation. The second model describes that NPR1 itself perceives SA, triggering an NPR1 conformational change thereby activating SA-mediated transcription. Besides the direct SA binding, NPR1 is regulated by SA-mediated redox changes and phosphorylation. Emerging evidence show that pathogen virulence effectors target SA signaling, further strengthening the importance of SA-mediated immunity

INTRODUCTION

THE BIOSYNTHESIS OF SA IN PLANTS

Findings

CONCLUSIONS AND PERSPECTIVES