Abstract

The objective of this study was to evaluate the physiological quality of commercial soybean seeds submitted to different concentrations of salicylic acid (SA), directly on germination paper and gerboxes for 24 hours. Seeds of cultivars NA 5909 RG and Tec Irga 6070 RR were soaked in salicylic acid solutions with concentrations of zero, 250, 500, 750, 1000, 1500, 2000, 3000, 4000 and 5000 μM. Seed imbibition occurred in two ways: (1) germination paper moistened with salicylic acid solutions; (2) imbibition of the seeds in salicylic acid solutions in gerbox boxes for 24 hours and subsequent sowing on germinated paper moistened with distilled water. On the fifth day after sowing, the number of normal seedlings (first count), length, fresh and dry mass of root and shoot were determined. It was concluded that the concentrations of SA between 250 and 750 μM can be used in soybean seeds, however, above 1000 μM may impair the parameters of physiological quality. The gerbox method for 24 hours provided the best results without the drastic reduction of the parameters in the lowest concentrations of SA.

Highlights

  • Soybean cultivation is of global importance in agribusiness because it is used for different objectives, both for animals and humans (Fioreze et al, 2011)

  • Commercial soybean seeds of Nidera® NA 5909 RG and Bayer®/Tec Irga 6070 RR cultivars produced in the 2015/2016 crop were used, both of which are recommended for cultivation in the state

  • Physiological quality of soybean seeds submitted to salicylic acid (SA) concentrations: after the initial characterization, the seeds were submitted to germination under different concentrations of SA (Sigma-Aldrich®): zero; 250; 500; 750; 1000; 1500; 2000; 3000; 4000; 5000 μM

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Summary

Introduction

Soybean cultivation is of global importance in agribusiness because it is used for different objectives, both for animals and humans (Fioreze et al, 2011). Salicylic acid (SA) is considered a plant hormone present in numerous plant species and acts in important biochemical and physiological processes, such as growth and development, stomatal closure, nutrient absorption, chlorophyll and protein synthesis, foliar abscission and seed germination (Hayat, 2010; Miura & Tada, 2014; Vazirimehr & Rigi, 2014). It has an influence on the response to different types of stress, through chemical signaling in plant cells together with antioxidant complex enzymes (Janda et al, 2014; Parmoon et al, 2017). Other enzymes such as superoxide dismutase, peroxidases, catalases, among others, have the function of reducing the damage caused by free radicals, such as reactive oxygen species (ROS), which are formed mainly in moments of stress (Hayat et al, 2012; Lee, Kim, & Park, 2010)

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